Performance and transcriptome analysis of Salmonella enterica serovar Enteritidis PT 30 under persistent desiccation stress: Cultured by lawn and broth methods.

Lawn-harvest method uses a solid medium (e.g., tryptic soy agar, TSA) to produce bacterial lawns and is widely accepted for the culture of microorganisms in microbial studies of low-moisture foods (LMFs, foods with water activity less than 0.85). It produces desiccation-tolerant cells with higher D-values in LMFs; however, little is known about the molecular mechanisms underlying bacterial resistance. Salmonella enterica Enteritidis PT 30 (S. Enteritidis), the most pertinent pathogen in LMFs, was cultured in TSA and tryptic soy broth (TSB). Cells were harvested and inoculated on filter papers to assess their performance under a relative humidity of 32 ± 2%. Transcriptome analysis of cultured cells during long-term desiccation (24, 72, and 168 h) was conducted in TruSeq PE Cluster Kit (Illumina) by paired-end methods. Lawn-cultured S. Enteritidis cells have stronger survivability (only decreased by 0.78 ± 0.12 log after 130 d of storage) and heat tolerance (higher D/ß value) than those from the broth method. More desiccation genes of lawn-cultured cells were significantly upregulated from growth to long-term desiccation. Differentially expressed genes were the most enriched in the ribosome and sulfur metabolism pathways in the lawn- and broth-cultured groups. This study tracked the transcriptomic differences between two cultured groups in response to long-term desiccation stress and revealed some molecular mechanisms underlying their different suitability in microbial studies of LMFs.

Performance of digital servos in an optical frequency transfer network.

We demonstrate the use of three kinds of flexible digital servos for the stabilization of the optical fiber link, the interferometer temperature, and the polarization of the transmitted light at the remote site, respectively. The main fiber noise cancellation digital servo provides a large phase detection range (∼210π radians), automatic relock function, and low cycle-slip rate over a 62 km field-deployed fiber link achieved by utilizing a feedback optical actuator of an acousto-optic modulator fed by a voltage-controlled oscillator. The temperature control and polarization control digital servos enable that the temperature of the interferometer can be stabilized at a stability of 0.01 K and the data uptime is enhanced from 85.5% to 99.9% by implementing the polarization controller. The results demonstrate that the performance of the three digital servos is sufficient for high-precision optical frequency transfer applications and indicates comparable performance to existing analog optical frequency control systems. The full digital controlled optical frequency transfer method demonstrated here provides guidance for the development of a low-cost, low-complexity, and high-reliability optical frequency transfer system.

All-passive multiple-place optical phase noise cancellation.

We report on the realization of delivering coherent optical frequency to multiple places based on passive phase noise cancellation over a bus topology fiber network. This technique mitigates any active servo controller on the main fiber link and at arbitrary access places as opposed to the conventional technique, in which an active phase compensation circuit has to be adopted to stabilize the main fiber link. Although the residual fiber phase noise power spectral density in the proposed technique turns out to be a factor of seven higher than that of in the conventional multiple-access technique when the access place is close to the end of the fiber link, it could largely suppress the phase noise introduced by the servo bumps, improve the response speed and phase recovery time, and minimize hardware overhead in systems with many stations and connections without the need for active servo circuits including phase discriminators and active compensators. The proposed technique could considerably simplify future efforts to make precise optical frequency signals available to many users, as required by some large-scale science experiments.